FIG 2.

Malnutrition and fecal-oral contamination promote fatty liver features. (A) Representative whole liver (left) and H&E-stained liver histology (right). Malnourished livers exhibit microvesicular steatosis (hepatocyte lipid accumulation) and macrovesicular steatosis (hepatocyte lipid accumulation characterized by a large fat vacuole and displaced cell nucleus). Representative examples of microvesicular steatosis and macrovesicular steatosis are adjacent to the black chevron or double chevron, respectively. (B) Percent of fat-associated space (open spaces) in liver histology images, assessed with ImageJ software. Each point represents a biological sample. (C) Triglyceride level normalized to liver weight. Data were pooled from three experiments with triglyceride levels normalized to the CON group of each experiment. Bars indicate means ± SEM. Statistical significance was determined by Kruskal-Wallis with post hoc Dunn’s test (histology) or one-way ANOVA with post hoc Dunnett’s test (triglycerides) and indicated as follows: **, P < 0.01; ****, P < 0.0001.