FIG 3.

Gene length can influence gene expression under translation limitation. (A) Change in GFP (238 aa) and β-galactosidase (1,024 aa) expressed from the P5 promoter as a function of increasing chloramphenicol concentration. The bacteria were grown in M9 with glucose plus cAA with chloramphenicol (Cm) at concentrations of 0, 2 μM, 4 μM, or 6 μM in flasks. The concentrations were normalized by dividing the data by the point without Cm. (B) Fit of the model to the ratio of GFP to β-galactosidase concentrations from the data in panel A. The growth rate at each chloramphenicol concentration is the average over the two strains. The error bars correspond to the SEM from three independent experiments.