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. 2020 Sep 10;10:14906. doi: 10.1038/s41598-020-72023-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Infarct volumes and neuronal nuclear (NeuN) positive (+) cells (A) Infarct volumes at 24 h after ischaemia were measured in the nearest eight slices of T2-weighted imaging (T2-WI) on stereotactically predetermined coronal planes. (B) The infarct volumes in all slices were the lowest in the TMF10 group, followed by the TMF50 and control groups. (C) Total infarct volumes were significantly lower in the TMF treatment groups than in the control group. Data are represented as means ± SDs (n = 8 rats in each group). (D) Representative NeuN staining (magnification × 630, scale bar = 20) from the IC (red square) and PIR (green square) in (A). (E,F) Quantification of the effect of TMF treatment on NeuN + cells in the (E) IC and (F) PIR. Data are represented as means ± SDs (n = 4 rats in each group). *P < 0.05 vs. sham group; **P < 0.001 vs. sham group; ^#P < 0.05 vs. control group; ^##P < 0.001 vs. control group; ^†P < 0.05 vs. TMF50 group.