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. 2020 Sep 10;10:14906. doi: 10.1038/s41598-020-72023-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Reduction in apoptosis after TMF treatment (A) Representative photomicrographs of terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) staining in the IC and PIR (magnification × 200, scale bar = 100). The right row shows enlarged versions of the black boxes in the middle row (scale bar = 20 μm). Red arrows indicate apoptotic cells; blue arrows indicate viable cells. (B,C) Quantification of the effect of TMF treatment on TUNEL positive (+) cells (%) in the (B) IC and (C) PIR. Data are represented as means ± SDs (n = 4 rats in each group). *P < 0.05 vs. control group; **P < 0.001 vs. control group.