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. 2020 Aug 28;11:1928. doi: 10.3389/fimmu.2020.01928

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Copyright © 2020 Batra, Dagar, Nayak, Kumaresan, Kumar and Datta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) The in situ removal of O- and N-linked glycan moieties by O-glycosidase along with α2-3,6,8,9 Neuraminidase A (O-Gly and Neu) and PNGase, respectively. (B–D) Bar graphs of the mean fluorescent intensity (MFIs) values obtained after quantification of the fluorescent signal produced during LCC (lectin cytochemistry) experiments using ImageJ. The signal was produced upon binding of ABL (B), JAC (C), and LEL (D) on the HF and enzyme-treated (Deglycosylated) HF bull spermatozoa. The differences in MFI were assessed by paired two-tailed t-test. O-Deglycosylated HF, High fertile bull spermatozoa treated with O-glycosidase along with α2-3,6,8,9 Neuraminidase A, N-Deglycosylated HF, High fertile bull spermatozoa treated with PNGase F. *P < 0.05 and **P < 0.01.