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. 2020 Aug 28;11:962. doi: 10.3389/fgene.2020.00962

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Copyright © 2020 Ding, Xi, Wang, Cui, Zhang, Wang, Jiang, Song, Xu and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Circ-MEMO1 silencing restrains cell proliferation and cell cycle progression and triggers cell apoptosis in NSCLC cells. (A–I) A549 and H1299 cells were transfected with si-NC or si-circ-MEMO1. (A) Circ-MEMO1 level was detected in transfected NSCLC cells by qRT-PCR. (B,C) MTT assay was utilized to analyze the proliferation of NSCLC cells. (D–G) Flow cytometry was used to analyze the percentage of NSCLC cells in G0/G1, S, or G2/M phase with the silencing of circ-MEMO1 or not. (H,I) The percentage of apoptotic NSCLC cells in the early stage and late stage was analyzed by flow cytometry. *P < 0.05.