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. 2020 Aug 28;11:962. doi: 10.3389/fgene.2020.00962

FIGURE 7.

FIGURE 7

MiR-101-3p directly interacts with the 3′ UTR of KRAS in NSCLC cells. (A) The interactions of miR-101-3p-mRNAs were screened by StarBase software, and KRAS was a candidate target of miR-101-3p. The mutant binding sites with miR-101-3p in KRAS were also shown. (B,C) Dual-luciferase reporter assay was performed to test the direct interaction between miR-101-3p and KRAS in NSCLC cells. (D,E) The regulatory relationship between miR-101-3p and KRAS in NSCLC cells was analyzed through transfecting miR-101-3p, anti-miR-101-3p, and their negative controls into NSCLC cells, and then the expression of KRAS was measured via Western blot assay. (F,G) The expression of KRAS was detected in A549 and H1299 cells co-transfected with si-NC + anti-NC, si-circ-MEMO1 + anti-NC, or si-circ-MEMO1 + anti-miR-101-3p by Western blot assay. (H) The expression of KRAS mRNA in NSCLC tumor tissues and matching normal tissues was examined by qRT-PCR assay. (I) Western blot assay was used to detect the protein expression of KRAS in NSCLC tumor tissue and matching normal tissue. (J) The expression of KRAS in HBE, H1299, and A549 cell lines was detected by Western blot assay. *P < 0.05.