Skip to main content
. 2020 Aug 28;11:2028. doi: 10.3389/fimmu.2020.02028

FIGURE 2.

FIGURE 2

CXCR4 overexpression on NK cells. Primary NK cells were transduced with huCAR19.CXCR4-LV or huCAR19-LV particles and analyzed for CXCR4 expression by flow cytometry 3 days post-transduction. Untransduced (unt) cells were cultured and analyzed in parallel. Each transduction experiment was performed at least 10 times with technical replicates. Three to four individually produced vector stocks were used. Mean value, SD, and significance are indicated. (A) Surface expression of CXCR4 on CD56-positive NK cells. Representative dot plots (upper panel) indicate the percentage of cells in each quadrant. Individual results of each experiment for CD56, CXCR4-double positive cells are shown in the lower panel. (B) Surface expression of CXCR4 on CD19-CAR-positive NK cells. Representative dot plots (upper panel) indicate the percentage of cells in each quadrant. Individual results of each experiment for CXCR4, CAR-double positive cells are shown in the lower panel. ****p < 0.0001; ns, not significant by unpaired t test. Characteristics of isolated, primary NK cells prior to transduction are shown in Supplementary Figure S1.