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. Author manuscript; available in PMC: 2020 Sep 11.
Published in final edited form as: Essays Biochem. 2020 Sep 4;64(2):289–297. doi: 10.1042/EBC20190077

Figure 1. Mechanisms explaining how Sgo1 regulates centromeric cohesion during mitosis.

Figure 1.

In prophase, Bub1-phosphorylated H2A recruits Sgo1 to kinetochores. RNA Polymerase (RNAP) II transcription promotes the relocation of kinetochore Sgo1 to the inner centromere, where Cdk1-phosphorylated Sgo1 binds to cohesin to protect centromeric cohesion. Cohesin on chromosome arms is released with the aid of multiple mitotic kinases. At metaphase-to-anaphase transition, SET removes Sgo1 from inner centromeres to de-protect centromeric cohesion by disrupting either the Sgo1–cohesin or Sgo1-nuclosome binding or both. SET may also inhibit PP2A activity to de-protect centromeric cohesion. It is unknown whether Separase cleavage of centromeric cohesin requires Sgo1 removal from inner centromeres (marked by “?”).