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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Clin Cancer Res. 2020 Jun 22;26(17):4448–4453. doi: 10.1158/1078-0432.CCR-20-0358

Figure 1: Differential modulation of B7-H3 and CSPG4 tumor antigen expression on cancer cells cultured under TME-like conditions.

Figure 1:

Cancer cell lines (Triple negative breast cancer, SUM159; Squamous cell carcinoma of the head and neck, PCI-13 and UMSCC-11B; Cholangiocarcinoma, ICC-3) were cultured under TME-like conditions (1% oxygen, pH6.5, adenosine IC5) for six days. Cells cultured under normoxia in unmodified media (‘normal’ in vitro conditions) were used as controls. After a six day culture at 37°C cells were harvested and stained using the B7-H3 mAb 376.96 (A) and the CSPG4 mAb 225.28 (B). Stained cells were then analysed by flow cytometry for antigen expression level. MFI comparison between the normal and TME-like conditions is shown. Error bars represent standard deviation.