Table 1:

Examples of conventional in vitro effector-target co-culture assays

Assay Type	Examples	Summary
Cell Viability	Chromium release	Radioactively labelled target cells release chromium when lysed
	MTT, XTT, ATP assays	Metabolically activated target cells produce substrate detected using a spectrophotometer
	Live/dead	Fluorescently labelled or transfected target cells measured by FACS or microscopy analysis
Cytokines	IFN-gamma expression and release	Cytokine release by effector cells measured by ELISPOT/ELISA
Apoptosis	Annexin V and 7-AAD/PI	Binding to phosphatidylserine and DNA respectively to detect early and late apoptosis by FACS
	TUNEL assay	Labelling of fragmented DNA produced during apoptosis
Degranulation	CD107a assay	Detection of cytotoxic granule marker at the extracellular membrane of effector cells