Table 1.
Antibody | Dilution and application | Source | Cat # |
---|---|---|---|
Mouse monoclonal α-SMA-FITC conjugated | 1:200 ICC | Sigma | F3777 |
Rabbit α-SMA | 1:500 WB 1:100 IHC |
Abcam | Ab5694 |
Mouse monoclonal GFAP | 1:500 WB 1:100 ICC |
Thermo Fisher |
MA5-12023 |
Mouse monoclonal NCAM | 1:100 ICC | Sigma | C9672 |
Rabbit s100β | 1:100 ICC | Abcam | Ab52642 |
Rabbit Laminin | 1:100 ICC | Sigma | L9393 |
Anti-Mouse HRP | 1:1000 WB | Cell Signalling |
7076S |
Anti-Rabbit HRP | 1:1000 WB | Cell Signalling |
7074S |
Donkey Anti Mouse 594 | 1:200 ICC | Invitrogen | A-21207 |
Donkey Anti- Rabbit 488 | 1:200 ICC | Invitrogen | A-21206 |
Mouse monoclonal GFAP | 1:100 IHC | NeoMarkers | Ab-6 |
ICC=immunocytochemistry, IHC=immunohistochemistry, WB=western blot