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. 2020 Aug 28;11:570985. doi: 10.3389/fmicb.2020.570985

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Copyright © 2020 Long, de Vries and Venema.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Average cumulative short chain fatty acid (SCFA) production in mmol (A) and SCFA increase in mmol/g substrate/24 h (B) for non-processed RSM (CON) and RSM processed by Accellerase 1000 (CELL), Pectinex Ultra SP (PECT1), Multifect Pectinase (PECT2), or 6 M NaOH (ALK). The values at time point 0 were artificially set to 0 to allow determination of cumulative production. (C) correlation between SCFA production and core genera, *p < 0.05; blue: positive correlation; red: negative correlation. Core genera: the table was normalized via division by the sum of sequences in a given sample and multiplied by the minimum sum across all samples. Relative abundances were filtered as follows: values below a relative abundance threshold of 0.01% were not taken into account; taxa with a median relative abundance < 1% in all groups were not considered for statistical analysis.