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. Author manuscript; available in PMC: 2021 Sep 3.
Published in final edited form as: Mol Cell. 2020 Jul 9;79(5):836–845.e7. doi: 10.1016/j.molcel.2020.06.028

Figure 5: MacroH2A1 variants predict DSB repair pathway choice in tumor cells.

Figure 5:

(A) Relative abundance of macroH2A1.2 splice variant expression in NCI60 tumor cell lines based on RNA-Seq expression analysis in CellMinerCDB. (B, C) Correlation of macroH2A1 splice variant expression with genes involved in genome maintenance pathways across NCI-60 tumor cell lines. Genes associated with HR and replication pathways (B) or end joining (EJ) pathways (C) are shown. Heat maps represent p values of the expression correlation, significance was assessed by two-tailed Z test. (D) Western blot using two NCI60 cell lines with high or low macroH2A1.1 or macroH2A1.2 levels. (E) Relative efficiency of alt-EJ in MCF-7 and Hs-578T cells with KD of macroH2A1.1 or macroH2A1.2. ** p < 0.01, *** p < 0.001 based on Student’s two-tailed t-test. (F) Western blot showing siRNA mediated knockdown of macroH2A1.1 and macroH2A1.2 cells from (E). See also Fig. S4.