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. 2020 Sep 10;10:14945. doi: 10.1038/s41598-020-71966-z

Figure 5.

Figure 5

Effects of 3-AP and H. erinaceus administration on the morphological changes of Purkinje cells by haematoxylin and eosin staining. Notably, animals in the 3-AP + 10 mg/kg H.E. (A) and 3-AP + 25 mg/kg H.E. groups (B) showed Purkinje cells with regular and ordinary morphology that was similar to the non-3-AP control group. Interestingly, Purkinje cells in the 3-AP + saline group (C) were scattered with disturbed alignment and irregular appearance. The Purkinje cells in the non-3-AP control group (D) showed regular morphology and ordinary alignment between granular and molecular layers. The scale bars represent 50 μm. The Purkinje cell linear density of 3-AP + 10 mg/kg H.E. (1752 Purkinje cells per 58,729.942 μm total PC length, 30 sections from 6 animals), 3-AP + 25 mg/kg H.E. groups (1519 Purkinje cells per 50,799.906 μm total PC length, 25 sections from 5 animals), and the non-3-AP control group (1,295 Purkinje cells per 42,788.036 μm total PC length, 20 sections from 4 animals) showed significant differences compared to 3-AP + saline group (1,348 Purkinje cells per 51,171.87 μm total PC length, 25 sections from 5 animals) (E). Interestingly, there was a remarkable reduction of caspase-3 in 3-AP + 25 mg/kg H.E. animals compared to the 3-AP + saline group, indicating the neuroprotective effects of 25 mg/kg of H. erinaceus against apoptosis (F). A significant decrease of gene expression for PCP4 (G) and calbindin-D28k (H) was found in 3-AP + 25 mg/kg H.E. group compared to the 3-AP + saline and the non-3-AP control groups. Indicators: * Significantly different from the non-3-AP control group. # Significantly different from the 3-AP + saline group. p values < 0.05.