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. 2020 Sep 10;3:500. doi: 10.1038/s42003-020-01235-2

Fig. 6. In situ hybridization and RT-qPCR of Ovule 4-, Stamen 5-, and Petal-enriched genes.

Fig. 6

a Longitudinal sections of pineapple stage 4 ovules with antisense and sense probes to detect Aco005999 (encoding chaperone protein ClpB), Aco008359 (encoding a MADS-box transcription factor), and Aco001460 (encoding a 70 kDa heat shock protein). Bars = 20 μm. b Cross-sections of pineapple stage 5 stamens with antisense and sense probes to detect Aco005285 (encoding a class I glutamine amidotransferase-like superfamily protein), Aco008952 (encoding an aquaporin-like superfamily protein), and Aco002322 (encoding a sequence-specific DNA binding transcription factor). Bars = 100 μm. c Validation of nine random selected petal specific subtilase genes by RT-qPCR. The left y-axis represents for the relative expression of RT-qPCR result and right y axis stands for the FPKM value from RNA-seq result. Blue dash line represents for RT-qPCR and red solid line represents for RNA-seq result. The letter P1–P3 means petal tissues at different stages.