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. 2020 Sep 10;11(9):737. doi: 10.1038/s41419-020-02965-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Immunofluorescence staining of in vitro cultured mouse ovaries after miR-378-3p overexpression. Germ cells were stained red with anti-Mvh antibody, nuclei were stained blue with Hochest 33342; b The total number of germ cells after miR-378-3p overexpression; c The percentage of germ cells in Cyst and primordial follicles after miR-378-3p overexpression; d The expression of miR-378-3p after miR-378-3p overexpression by miRNA qRT-PCR; e–h Protein expression of autophagy-related genes (LC3B, PDK1, mTOR and p-mTOR) by western blotting; i Autophagic flux after Bafilomycin A1 (BA1) treatment; j–l Protein expression of apoptosis-related genes (BAX, BCL-2, CASP9 and CASP3) by western blotting.