A) ChAT-immunoreactive tissue sections from the distal colon control (top), 3-day T3-SCI (middle) or 3-week T3-SCI (bottom). Tissues sections are from separate distal colon samples harvested from rats used for intracellular recordings (X400, scale bar, 50 μm). B) ChAT-positive neurons were reduced following T3-SCI in the distal colon (Values expressed as mean ± SEM, *p < 0.05 vs. post-operative time-matched controls, n = 24).