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. Author manuscript; available in PMC: 2021 Aug 15.
Published in final edited form as: Eur J Pharmacol. 2020 May 31;881:173233. doi: 10.1016/j.ejphar.2020.173233

Fig.1. Mice with NAC treatment exhibited enhanced recovery of blood flow and limb function after limb ischemia.

Fig.1.

Limb ischemia was induced in C57BL/6 mice with or without NAC treatment by left hind limb femoral artery ligation. Mouse hind limb blood flow was measured using LDPI before, after ligation, and at day 3, 7, 14, and 21 after limb ischemia procedure. Mouse limb function was evaluated using the running time on treadmill at baseline and at day 7, 14, and 21 after limb ischemia. No measurable blood flow was present in the limb immediately after femoral artery ligation (A), confirming successful creation of the limb ischemia model. The recovery of blood flow (B) and limb function (C) was significantly enhanced in mice with NAC treatment compared to the control. NAC: C57BL/6 mice with NAC treatment; Ctrl: C57BL/6 mice with PBS control. Ischemic limb/normal limb: ratio of mouse ischemic left hind limb blood flow to normal right hind limb blood flow. *Day14 or 21 vs day7, P <0.01, n=8; #ctrl vs NAC, P <0.01, n=8.