Murine BM and blood cells were collected to determine the proliferation rate at day3 and 7 using flow cytometry analysis. Mice without limb ischemia were used as control. There was no significant change in the BM cell proliferation rate at day 3 and day 7 after limb ischemia. However, the proliferation rate of blood mononuclear cells were significantly decreased at day 7 following limb ischemia. When the mice were pre-treated with NAC, there was no significant change in the BM mononuclear cell proliferation rate after limb ischemia compared with the control. At day 21, the blood mononuclear cell proliferation was preserved to the same level as day 3 and 7 in NAC-treated mice, which was much higher than that in the control. *Day3 or 7 vs Baseline, P <0.05, n≥8; $Day7 vs 3, P <0.05, n≥8; #ctrl vs NAC, P<0.05, n≥8.