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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Obesity (Silver Spring). 2020 Aug 2;28(9):1726–1735. doi: 10.1002/oby.22912

Figure 1. SCO inhibits TNFα-induced expression of inflammatory genes in 3T3-L1 adipocytes.

Figure 1.

Mature 3T3-L1 adipocytes were pretreated with 50 μg/ml SCO for 3-days, then with 0.75 nM TNFα for 18 hours. Cells were harvested for RNA isolation, and gene expression was assayed by qPCR. Target gene data were normalized to the reference gene Nono. Fold-change was calculated versus the mean of TNFα-only controls for each gene. Data are shown as means +/− SE; n=4 biological replicates per condition. N.D.: Nos2 was below detection level for the qPCR assay. Data for each gene were analyzed by one-way ANOVA (for Ccl2, Il6, and Lcn2) or t-test (for Nos2). Significance expressed as ***P<0.001, ****P<0.0001 vs. TNFα-only controls. Results were replicated in two additional experiments on independent batches of cells.