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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Obesity (Silver Spring). 2020 Aug 2;28(9):1726–1735. doi: 10.1002/oby.22912

Figure 3. SCO inhibits TNFα-induced cellular levels and secretion of LCN2 in adipocytes.

Figure 3.

Mature 3T3-L1 adipocytes were pretreated with 50 μg/ml SCO or DMSO vehicle for 3 days prior to harvest. On the last day of SCO treatment, cells were treated with 0.75nM TNFα or 0.1% BSA vehicle for 18 hours. Medium on all cells was then replaced with low-glucose phenol-red-free DMEM containing either vehicle or 0.75 nM TNFα. After 4 hours, samples of conditioned media were collected and cells were harvested in RIPA buffer for immunoblotting. 125 μg (media) or 75 μg (whole-cell extracts) of total protein were loaded in each well.