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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Obesity (Silver Spring). 2020 Aug 2;28(9):1726–1735. doi: 10.1002/oby.22912

Figure 4. SCO reduces total and phosphorylated ERK levels in 3T3-L1 adipocytes.

Figure 4.

Mature 3T3-L1 adipocytes were pretreated with 50 μg/ml SCO or DMSO vehicle for 3 days, with 18-hour (overnight) serum deprivation (0.3% BSA) and 0.5 nM TNFα treatment on the last day. Cells were harvested in RIPA buffer and analyzed by Western blot. Blot images and quantitation data are shown. *P<0.05 and ***P<0.001 for effect of SCO vs. respective controls (CON or TNFα only); #P<0.05 for effect of TNFα vs CON; NS: not significant. Results were replicated in two additional experiments on independent batches of cells.