Figure 5. SCO reduces nuclear DBC1 and TNFα-induced p65 nuclear translocation in 3T3-L1 adipocytes.

Mature 3T3-L1 adipocytes were pretreated for 3 days with 50 μg/ml SCO or DMSO vehicle, then with 0.5 nM TNFα for 20 minutes. Cells were harvested, and the cytosolic and nuclear compartments were isolated. Immunoblotting was performed to detect DBC1, p65 phosphorylated at Ser536. ERK 1/2 was included as a loading control for the cytoplasmic fractions.