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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Obesity (Silver Spring). 2020 Aug 2;28(9):1726–1735. doi: 10.1002/oby.22912

Figure 6. SCO does not reduce TNFα- or isoproterenol-induced FABP4 secretion.

Figure 6.

Mature 3T3-L1 adipocytes were pretreated with 50 μg/ml SCO or DMSO vehicle for 3 days prior to harvest. On the last day of SCO treatment, cells were treated with 0.75nM or 0.1% BSA vehicle overnight (18 hours). The following day, medium on all cells was replaced with low-glucose phenol-red-free DMEM containing either vehicle, 0.75 nM TNFα, 2 nM or 10 μM isoproterenol (ISO). Samples of conditioned media were collected after 4 hours. (A) 75 μg of total protein were loaded in each well and analyzed with standard immunoblotting techniques. Membranes were probed for FABP4. Blot images are shown. WCE: whole-cell extract control lysate. (B) 50 μl of conditioned media were also assayed for glycerol content. Effects of TNFα and isoproterenol versus control in the DMSO condition were analyzed by one-way ANOVA: ## P<0.01; #### P<0.0001 vs DMSO control. The effect of SCO was assessed in each condition versus the respective DMSO condition by t-tests. * P<0.05. All other interactions were non-significant.