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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Obesity (Silver Spring). 2020 Aug 2;28(9):1726–1735. doi: 10.1002/oby.22912

Figure 8. SCO inhibits IL-1β-stimulated NF-κB promoter activity in rat insulinoma cells.

Figure 8.

832/13 cells were transduced with an adenovirus overexpressing a luciferase reporter construct driven by a promoter containing 5 copies of a consensus κB element (5xNF-κB-Luc). 12h post-transduction, cells were untreated, or treated overnight with 5 and 10 μg/mL of SCO or an extract of Artemisia santolinaefolia (SAN). Cells were then stimulated for 4h with 1 ng/mL IL-1β. NF-kB promoter activity was induced 28.6-fold (for the SCO experiment) or 31.1-fold (for the SAN experiment) by IL-1b treatment (not shown). Promoter activity in cells pretreated with the extracts is expressed as % of this maximal induction. Data are means ± SEM of 4 individual experiments, each performed in triplicate. **, P < 0.01 versus control.