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. 2020 Aug 28;11:1998. doi: 10.3389/fmicb.2020.01998

Figure 2.

Figure 2

TcdA downregulates β-catenin target genes (cMYC and cyclin D1) and reduces cyclin D1 protein in the ileum of the treated mice. (A) CCND1 and (B) cMYC mRNA expression in the ileum of mice treated with phosphate-buffered saline (PBS; control) or TcdA (10 μg) for 4 h, as evaluated by qPCR. Bars represent the means ± SEM of five mice in each group; Student’s t-test. (C) Percentage of the cells showing positive cyclin D1 immunostaining. Data are the means ± SEM; Student’s t-test. (D) Representative immunohistochemical images of cyclin D1 levels in the ilea of mice challenged with PBS (control) or TcdA (10 μg). (E) Percentage of the cells showing positive cMYC immunostaining. Data are the means ± SEM; Student’s t-test. (F) Representative immunohistochemical images of cMYC expression in mouse ilea challenged with PBS (control) or TcdA (10 μg).