Figure 6.

Upregulation of Rac1 reverses the TcdA inhibitory effect on Wnt3a-induced proliferation of the IEC-6 cells. (A) Percentage of IEC-6 cells showing positive Ki67 immunostaining after being cotransfected with pcDNA (empty vector) or pcDNA3-EGFP-Rac1-Q61L upon incubation with TcdA (50 ng/ml), Wnt3a-CM or L-cell medium for 24 h. Data are the means ± SEM (n = 6). One-way ANOVA, followed by the Bonferroni’s test, was used; ^**p < 0.001. (B) Representative immunohistochemical images of the Ki67-immunostained IEC-6 cells transfected with pcDNA (empty vector) or pcDNA3-EGFP-Rac1-Q61L, followed by 24 h incubation with TcdA alone or Wnt3a-CM or L-cell medium.