TABLE 2.

Summary of hNLRP1, CARD8 and mNLRP1B binding to DPP9

Binding Interaction	hNLRP1‐DPP9	CARD8‐DPP9	mNLRP1B‐DPP9
Subdomains required	ZU5‐UPA	ZU5‐UPA	NT
Binding to catalytically inactive DPP9	Yes	Yes	NT
Binding of autoproteolysis deficient mutant a	Minimal	Yes	Yes
VbP displacement in vitro	Yes	No	No
Catalytically inactive DPP9 inhibits the inflammasome	Yes b	No c	NT

^aAutoproteolysis deficient mutants tested include: hNLRP1 F1212A and S1213A, CARD8 S297A, and mNLRP1B S984A.

^bStable expression of catalytically inactive DPP9 S759A partially rescued NLRP1‐ASC puncta formation in reconstituted DPP8/9^−/− HEK 293T cells. ⁶² , ⁶⁸

^cStable expression of DPP9 S759A did not rescue CARD8‐CASP1 mediated cell death in reconstituted DPP9^−/− HEK 293T cells. ⁶⁶