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. Author manuscript; available in PMC: 2021 Aug 20.
Published in final edited form as: Cell Chem Biol. 2020 Jul 21;27(8):1063–1072.e7. doi: 10.1016/j.chembiol.2020.07.001

Figure 3. Photoablation of neurons in larval zebrafish.

Figure 3.

(a) Experimental scheme of larval zebrafish neuronal ablation experiment. (b) Cartoon of larval zebrafish brain “zones” used in this experiment; yellow circle indicates approximate area of irradiation. (c) Representative fluorescence microscopy images of zebrafish pre-irradiation (t = 0 min), and one day post-irradiation (t = 24 h) expressing HaloTag fusions to the mitochondrial protein TOMM20 (magenta) co-expressing mCerulean (cyan) and incubated with either JF570–HaloTag ligand (10HTL, top; 3 W/cm2 excitation) or JF585–HaloTag ligand (20HTL, bottom; 12 W/cm2); scale bars: 100 μm. (d) Quantification of cell-ablation efficacy using JF570–HaloTag ligand (10HTL) with JF585–HaloTag ligand (20HTL) as control; n = 6 for 10HTL experiments and n = 5 for 20HTL experiments; center line indicates median; box limits indicate upper and lower quartiles; whiskers indicate min–max; data analyzed via independent t-tests. Statistical significance reported as follows: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. See also Figure S4.