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. Author manuscript; available in PMC: 2021 Jan 8.
Published in final edited form as: Nature. 2020 Jul 8;585(7823):141–145. doi: 10.1038/s41586-020-2480-9

Extended Data Fig. 1. Pharmacology and cryo-EM data collection and processing for TRPA1.

Extended Data Fig. 1.

a, All points histograms depicting the change in open probability (P(o)) in a single TRPA1 channel in response to IA-application. Data represent n = 9 independent excised inside-out patches. Vhold = −40 mV. b, SDS-PAGE showing MBP-TRPA1 (arrowhead) after pull-down and elution from amylose beads. c, Cryo-EM image of MBP-TRPA1. Scale bar: 20 nm. d, 2D classification of cryo-EM particle images showing TRPA1 in different orientations. Scale bar: 25 Å. e, Pharmacological agents used in this study.