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. Author manuscript; available in PMC: 2021 Jan 8.
Published in final edited form as: Nature. 2020 Jul 8;585(7823):141–145. doi: 10.1038/s41586-020-2480-9

Extended Data Fig. 2. Fourier shell correlation of cryo-EM maps, orientation distribution of particle image views, and local resolution of TRPA1 cryo-EM maps.

Extended Data Fig. 2.

a, Fourier shell correlation and 1D directional Fourier shell correlation plots. TRPA1 (PMAL) + A-967079 class 2 denotes the structure derived from 3D classification of antagonist-treated samples in PMAL and represents the open state channel without antagonist bound. b, 3D representations of the directional Fourier shell correlation. c, Fourier space covered, based on dFSC at 0.143. d, Orientation distribution of particle image refinement angles. e, The A-loop is lower resolution compared to surrounding map regions, indicating its dynamic nature. In the activated (TRPA1+iodoacetamide) and open state (TRPA1 + A967079 PMAL-C8 class 2) conformations, the bottom of S6 is lower resolution compared to surrounding regions, indicating structural flexibility at the level of the lower gate. Scale bars: 5Å.