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(a) Cryo-EM density (gray surface) and structure model (colored ribbon) of the 16-helix TMH4C4 protein. EM maps, generated in Chimera34, are shown in two perpendicular views. (b) Superposition of the cryo-EM structure (colored) and design model based on the crystal structure of the soluble form (gray) of TMH4C4. (c) Structure alignment of the four protomers in one tetrameric cryo-EM structure of TMH4C4.
