Extended Data Table 2 |.
Cryo-EM Image Processing Statistics for TMH4C4.
| TMH4C4 (EMD-30126) (PDB-6M6Z) |
|
|---|---|
| Data collection and processing | |
| EM equipment | Titan Krios (Thermo Fisher Scientific) |
| Magnification | 81,000 |
| Voltage (kV) | 300 kV |
| Detector | Gatan K3 Summit |
| Energy Filter | Gatan GIF Quantum, 20 eV slit |
| Electron exposure (e−/Å2) | 50 |
| Defocus range (μm) | −1.2 ∼ −2.2 |
| Pixel size (Å) | 1.087 |
| Initial particle images (no.) | 2,146,524 |
| Final particle images (no.) | 64,739 |
| Symmetry imposed | C1 |
| Map resolution (Å) | 5.9 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 5.2 – 7.2 |
| Refinement | |
| Initial model used (PDB code) | n/a (de novo) |
| Model resolution (Å) | 7.7 |
| FSC threshold | 0.5 |
| Map sharpening B factor (Å2) | −292 |
| Model composition | |
| Non-hydrogen atoms | 6600 |
| Protein residues | 804 |
| Ligands | n/a |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.016 |
| Bond angles (°) | 1.3 |
| B factors (Å2) | |
| Protein | 169.4 |
| Ligand | n/a |
| Validation | |
| MolProbity score | 0.52 |
| Clashscore | 0.05 |
| Poor rotamers (%) | 0 |
| Ramachandran plot | |
| Favored (%) | 99.63 |
| Allowed (%) | 100 |
| Disallowed (%) | 0 |