Skip to main content
. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Exp Eye Res. 2020 May 23;197:108079. doi: 10.1016/j.exer.2020.108079

Figure 3. S. aureus induced MEK/ERK pathways is inhibited by 2DG.

Figure 3.

BMDMs were pretreated with 2DG (10 mM) for 1 h followed by challenge with S. aureus (SA) for indicated time-points. Cells were harvested and subjected to Western blotting for pERK, ERK and HSP90 proteins (A). BMDMs were pretreated with either 2DG (10 mM) or ERK inhibitor U0126 (10 μM) for 1 h, followed by a challenge with SA for 8 h. Cells were harvested and subjected to Western blotting for pERK, ERK and HSP90 proteins (B). The conditioned media from 2DG and U0126 pretreated cells in the presence and absence of SA were subjected to ELISA (C). Data represent mean ± SD from three independent experiments. A one-way ANOVA with Tukey post hoc test was used to determine statistical significance. **p<0.01, ***p<0.001, ns: not significant.