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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Exp Neurol. 2020 Jun 17;331:113385. doi: 10.1016/j.expneurol.2020.113385

Table 1.

Cellular and Molecular Characteristics of Cultured Primary Endoneurial Endothelial Cells

Species Cellular Characteristics Molecular Characteristics
Rat

  • Spindle-like / Cobblestone appearance

  • Contact-inhibited monolayer

  • Diameter: 20.5 ± 3.2 μm

  • Proliferative (doubling time ~48h)

  • Factor VIII/von Willebrand factor positive

  • CD31 (PECAM) positive

  • Claudin-5, Occludin, JAM-1, ZO-1 positive

  • Thy 1.1 negative

  • TNF-α responsive CCL2, ICAM-1

  • TEER (transwell): ~15 Ω.cm2

  • Transendothelial migration competent
Cow

  • Spindle-like / Cobblestone appearance

  • Contact-inhibited monolayer

  • Proliferative

  • Factor VIII/von Willebrand factor positive

  • GLUT-1 positive

  • Gangliosides: GM1, GM3, GD1a, GD1b, GTb1, SLPG positive
Human

  • Spindle-like / Cobblestone appearance

  • Contact-inhibited monolayer

  • Diameter: 21.1 ± 4.3 μm

  • Proliferative (doubling time ~48h)

  • Factor VIII/von Willebrand factor positive

  • Scavenger-receptor positive

  • α-L-Fucose (UEA-1 lectin) positive

  • VCAM-1, CD34, P-selectin positive

  • ICAM-1, E-selectin negative

  • Claudin-1 & 5, Occludin, JAM-1, ZO-1 positive

  • Enzymes: AP, γ-GT positive

  • Carrier-Mediated Transporters: GLUT-1, LAT-1, p-glycoprotein, CRT, MCT-1 positive

  • TEER (continuous electric cell imp.): >100 Ω.cm2

  • Hydraulic Conductivity: 2.0 × 10−7 cm s−1 cm H2O−1