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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Dev Biol. 2020 Jul 3;465(1):1–10. doi: 10.1016/j.ydbio.2020.06.011

Figure 5: Notochord morphogenesis is disrupted in Ppp1r35 mutant embryos.

Figure 5:

Immunofluorescent analysis of laminin (green), FOXA2 (red), and nuclei counterstained with DAPI (blue). Consecutive sections through a stage-matched E8.5 control reveals a FOXA2-positive floor plate (boundary defined by dotted lines) and a contiguous notochord that has laminin distributed laterally and dorsally (A-A”). Although the two E8.75 two mutants vary slightly they both lack a FOXA2-positive floor plate and display altered notochord resolution (B-B”, C-C”). Fg; foregut. Ctrl denotes embryos wild type or heterozygous for Ppp1r35. −/− denotes Ppp1r35 homozygous embryos. Boxes indicate a higher magnification of the notochord. FOXA2-labeled mutant embryos: n=3. Scale bars = 20 μm.