Single nucleotide polymorphism in Spp2 leads to lower systolic blood pressure in male and lower bone volume in female rats. A) DNA sequencing results from the PCR products for both Spp12 non-founder and knock-in at the location Chr9:95506318 (Rnor 6.0) within exon 4 of Spp2. B) Radiotelemetry systolic BP measurements of Spp2 non-founder (N=8) vs. Spp2 knock-in (N=7) males on the left and Spp2 non-founder (N=8) vs. Spp2 knock-in (N=8) females on the right. Spp2 non-founder- red and Spp2 knock-in- blue. *P<0.05, **P<0.01. Proximal tibia trabecular bone BV/TV from Spp2 non-founder vs. Spp2 knock-in (N=3) in males (C) and females (E) *P< 0.05. 3D renderings of trabecular bone in proximal tibia from Spp2 non-founder and knock-in (N=3) in males (D) and females (F). Bar on micro CT renderings represents 1 mm.