| Antibodies |
| Goat anti-mouse TROP2-APC |
R&D |
Cat# AF1122; RRID:AB_2205662 |
| Rat anti-mouse ITGA6-PE |
BioLegend |
Cat# 313611; RRID:AB_893374 |
| Rabbit Keratin 5 |
Covance |
Cat # PRB-160P; RRID:AB_291581 |
| Chicken Keratin 5 |
Covance |
Cat# SIG-3475; RRID:AB_10720202 |
| Rat Keratin 8 |
DSHB |
Cat# TROMA-I; RRID:AB_531826 |
| Mouse GSK3β |
Abcam |
Cat# ab93926; RRID:AB_10563643 |
| Mouse Acetylated β-Tubulin |
Sigma |
Cat# T7451; RRID:AB_609894 |
| Rat BrdU |
Abcam |
Cat# ab6326; RRID:AB_305426 |
| Rabbit Porcupine |
Abcam |
Cat# ab105543; RRID:AB_10860951 |
| Mouse Porcupine |
Millipore-Sigma |
Cat# MABS21; RRID:AB_11203317 |
| Rabbit Laminin-A |
ThermoFisher |
Cat# PA5–16287; RRID:AB_10985513 |
| Rabbit Total β-catenin |
Abcam |
Cat# ab32572; RRID:AB_725966 |
| Mouse p-β-cateninY489
|
DSHB |
Cat# PY489-B-catenin; RRID:AB_10144551 |
| Rabbit p-β-cateninS33,S37,T41
|
Cell Signaling Technology |
Cat# 9561T; RRID:AB_331729 |
| Rabbit p-β-cateninS675
|
Cell Signaling Technology |
Cat# 4176; RRID:AB_1903923 |
| Chicken GFP |
Abcam |
Cat# ab13970; RRID:AB_300798 |
| Rat tdTomato |
Kerafast |
Cat# EST203; RRID:AB_2732803 |
| Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 |
ThermoFisher |
Cat# A-11037; RRID:AB_2534095 |
| Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 |
ThermoFisher |
Cat# A-11029; RRID:AB_138404 |
| Goat anti-Chicken IgY (H+L) Secondary Antibody, Alex Fluor 488 |
ThermoFisher |
Cat# A-11039; RRID:AB_142924 |
| Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 |
ThermoFisher |
Cat# A-21247; RRID:AB_141778 |
| Biological Samples |
| Human bronchiolar airway tissue specimens |
Ronald Reagan UCLA Medical Center |
N/A |
| Chemicals, Peptides, and Recombinant Proteins |
| Tamoxifen |
Sigma |
Cat# T5648 |
| BrdU |
Sigma |
Cat# B5002 |
| (Z)-4-Hydroxytamoxifen (4-OHT) |
Sigma |
Cat# H7904 |
| LGK974 |
MedChem Express |
Cat# HY-17545 |
| Dispase |
Corning |
Cat# 354235 |
| Collagen type I |
Corning |
Cat# 354249 |
| HEPES |
Sigma |
Cat# H0887 |
| Sodium Bicarbonate |
Life Technologies |
Cat# 25080–094 |
| L-glutamine |
Invitrogen |
Cat# 35050–061 |
| Insulin |
Sigma |
Cat# I6634 |
| Transferrin |
Sigma |
Cat# T5391 |
| Cholera Toxin |
Sigma |
Cat# C8052 |
| Epidermal Growth Factor (EGF) |
Corning |
Cat# 354001 |
| Bovine Pituitary Extract |
Invitrogen |
Cat# 13028–014 |
| Fetal Bovine Serum |
ThermoFisher |
Cat# SH3008803HI |
| Bovine Serum Albumin |
ThermoFisher |
Cat# BP9706 |
| Retinoic Acid |
Sigma |
Cat# R2625 |
| Polidocanol |
Sigma |
Cat# P9641 |
| Critical Commercial Assays |
| Click-iT EdU Incorporation Assay Kit |
Invitrogen |
Cat# C10337
|
| Mouse Wnt Signaling Pathway RT2 Profiler PCR Array |
Qiagen |
Cat# PAMM-043Z |
| Experimental Models: Organisms/Strains |
| Mouse: C57BL/6J |
The Jackson Laboratory |
JAX Stock# 000664 |
| Mouse: K5-CreERT2
|
The Jackson Laboratory |
JAX Stock# 029155 |
| Mouse: ROSAmT/mG
|
The Jackson Laboratory |
JAX Stock# 007576 |
| Mouse: Porcnfl/fl
|
The Jackson Laboratory |
JAX Stock# 020944 |
| Mouse: Pdgfrα-CreERTM
|
The Jackson Laboratory |
JAX Stock# 018280 |
| Mouse: Ctnnb1fl/fl
|
The Jackson Laboratory |
JAX Stock# 004152 |
| Mouse: K8-CreERT2
|
The Jackson Laboratory |
JAX Stock# 017947 |
| Mouse: TCF/Lef:H2B/GFP
|
The Jackson Laboratory |
JAX Stock# 013752 |
| Mouse: Ctnnb1LSL(Ex3)/+
|
Van Andel Institute, U.S. |
Kind gift from Mark Taketo via Bart Williams |
| Oligonucleotides |
| Primers for genotyping of mouse lines, see Table S1
|
This paper |
N/A |
| qPCR TaqMan probes, see Table S5
|
This paper |
N/A |
| Software and Algorithms |
| GraphPad Prism 7 |
GraphPad Software |
RRID:SCR_002798 |
| Fiji |
ImageJ |
RRID:SCR_002285 |
| Other |
| PLISH probe: Axin2
|
Nabhan et al. 2018 |
N/A |
