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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2020 Jul 2;40(9):2279–2292. doi: 10.1161/ATVBAHA.120.314491

Figure 3. The presence of gut commensals restricts the formation of neutrophil extracellular traps (NETosis) in ischemia-reperfusion injured mesenteric venules.

Figure 3.

(A) NETosis in mesenteric venules of GF and CONV-R mice (6 vs 13 mice/group) pre- and post-ischemia; adhering leukocytes were stained with acridine orange; NETs were visualized by SYTOX orange. For GF and CONV-R male and female mice were used. (B) NETosis in mesenteric venules pre- and post-ischemia in GF and CONV-D mice (5 vs 4 mice/group); adhering leukocytes were stained with acridine orange; NETs were visualized by SYTOX orange. For WT GF male mice and WT CONV-D male and female mice were used. (C) NETosis in mesenteric venules pre- and post-ischemia in CONV-R and (Abx)-treated CONV-R mice (13 vs 9 mice/group); adhering leukocytes were stained with acridine orange; NETs were visualized by SYTOX orange. For WT CONV-R and CONV-R (Abx)-treated male and female mice were used. (D) NETosis in mesenteric venules pre- and post-ischemia in GF and GF mice monoassociated with E.coli JP 313 (5 vs 5 mice/group); adhering leukocytes were stained with acridine orange; NETs were visualized by SYTOX orange. For GF male mice and GF monoassociated with E.coli JP 313 male and female mice were used. Results are shown as means ± s.e.m. Scale bar: 50 μm or 100 μm. Statistical comparisons were performed using the independent samples Studentś t-test (*) or the Mann-Whitney test (#), */#p<0.05, **/##p<0.01.