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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Free Radic Biol Med. 2020 Jul 23;158:149–161. doi: 10.1016/j.freeradbiomed.2020.07.006

Figure 2.

Figure 2.

(a) Absorption spectra recorded after the reaction of NH2α-Syn-Cu(II) (10 μM; black), NAcα-Syn-Cu(II) (10 μM; red), or free Cu(II) (10 μM; gray) with dopamine (1 mM) in 20 mM N-ethylmorpholine/100 mM NaCl, pH 7.4 at 25°C, in the presence of MBTH (2 mM). Auto-oxidation of dopamine (1 mM) is plotted in pink. (b) Concentration-dependent dopamine oxidase activity of NH2α-Syn-Cu(II) (10 μM; black) or NAcα-Syn-Cu(II) (10 μM; red) in 20 mM N-ethylmorpholine/100 mM NaCl, pH 7.4, determined using MBTH (2 mM) to quantify the dopamine ortho-quinone formed after 120 s reaction (25°C).