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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Free Radic Biol Med. 2020 Jul 23;158:149–161. doi: 10.1016/j.freeradbiomed.2020.07.006

Figure 5.

Figure 5.

(a) Concentration-dependent dopamine oxidase activity of soluble or membrane-bound NH2α-Syn-Cu(II) (10 μM; black and blue, respectively) and NAcα-Syn-Cu(II) (10 μM; red and green, respectively) in 20 mM N-ethylmorpholine/100 mM NaCl, pH 7.4, determined using MBTH (2mM) to quantify the dopaquinone formed. Values were obtained after 120 s reaction for soluble forms and 20 s for the membrane-bound forms (25°C). (b) Concentration-dependent dopamine oxidase activity of membrane-bound NAcα-Syn-Cu(II) (10 μM) in 20 mM N-ethylmorpholine/100 mM NaCl, pH 7.4 at increasing H2O2 concentrations (0 – 1 M), determined using 2 mM MBTH to quantify the dopaquinone formation (20 s, 25°C). (c) Michaelis-Menten analysis to determine Vmax, H2O2 as a function of H2O2 concentrations.