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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Free Radic Biol Med. 2020 Jul 23;158:149–161. doi: 10.1016/j.freeradbiomed.2020.07.006

Figure 7.

Figure 7.

(a) Catalytic dopamine (1 mM) oxidation by NH2α-Syn-Cu(II) or NAcα-Syn-Cu(II) (10 μM) in 20 mM N-ethylmorpholine/100 mM NaCl, pH 7.4, in the presence 2 mM MBTH (100 min, 25°C). The dopamine oxidase activity is quenched upon addition of Zn7MT-3 (0.25 eq., 1 h) prior to the addition of dopamine. (b) Quenching of the dopamine oxidase activity of membrane-bound NH2α-Syn-Cu(II) or NAcα-Syn-Cu(II) (10 μM) upon reaction with Zn7MT-3 (0.25 eq.). The dopaquinone formed was quantified after 20 s (25°C) using 2 mM MBTH.