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Michaelis-Menten analysis of the dopamine oxidase activity of soluble or membrane-bound NH2α-Syn-Cu(II) and NAcα-Syn-Cu(II) determined using MBTH (2 mM) in 20 mM N-ethylmorpholine/100 mM NaCl, pH 7.4.
| VMAX, DOPAMINE (nmol quinone*μmol−1α-Syn Cu(II)*min−1) |
KM, DOPAMINE (mM) | |
|---|---|---|
| NH2α -Syn-Cu(II) | 14.2 ± 2.5 | 0.30 ± 0.00 |
| NAcα-Syn-Cu(II) | 12.5 ± 0.6 | 0.23 ± 0.03 |
| mem NH2a-Syn-Cu(II) | 1094.0 ± 22.5 | 0.32 ± 0.03 |
| mem NAcα-Syn-Cu(II) | 1281.6 ± 63.6 | 0.29 ± 0.05 |
