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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Peptides. 2020 Jun 21;132:170350. doi: 10.1016/j.peptides.2020.170350

Figure 8. 14,15-EET causes an increase in BKCa channel current density in isolated cerebral smooth muscle cells.

Figure 8.

Whole cell BKCa currents were recorded in freshly isolated vascular smooth muscle cells in response to successive voltage pulses of 800 ms duration, increasing in 10 mV increments from −60 mV to +80 mV before and after treatment with 14,15-EET (10−6 M) with and without apelin (10−7 M) or Iberiotoxin (IBTx, 10−7 M). (A) Representative tracings depicting current recordings from a single smooth muscle cell before and after treatment with 14,15-EET (10−6 M, 2 min) alone, or with apelin (10−7 M, 5 min) or IBTx (10−7 M, 5 min); (B) Summary I-V curve plots of BKCa currents at baseline and after treatment with 14,15-EET (10−6 M) without and with apelin (10−7 M) or IBTx (10−7 M, 5 min); and (C) Bar graph summarizing the effect of apelin (10−7 M) or IBTx (10−7 M) on 14,15-EET (10−6 M)-induced average current density (pA/pF) at +80 mV. Values are presented as mean ± SEM (n=6). *p<0.05 vs. control current density; #p<0.05 vs. 14,15-EET-induced current density.