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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Free Radic Biol Med. 2020 Jul 26;158:181–194. doi: 10.1016/j.freeradbiomed.2020.06.039

Figure 6. Mitofilin mutation plasmids used to determine Mitofilin region that bind to CypD.

Figure 6.

A. Schematic representations of the structure of Myc-tagged Mitofilin mutants; 5: Full length (FL) Mitofilin, 4: Mitofilin-mutant 4, c-terminal (aa 476 – 757) deleted, 3: Mitofilin-mutant 3, c-terminal and C-C deleted (397 – 757) deleted, 2: Mitofilin-mutation2, (317 – 757) deleted, 1: Mitofilin-mutant 1 (279 – 757) deleted. B. Mutation efficiency of deletion in PCR mediated by high-fidelity DNA polymerase. C. Immunoblots showing the transfection efficiency of all the mutation plasmids in HEK293 cells.