eNOS is upregulated the cochlear lateral wall, spiral ganglion and
auditory nerve of
Ifnar1−/− ZIKV
infected mice. Baseline eNOS localization is seen in Type I and Type III
fibrocytes of the spiral ligament (sLg, A, B, E, F), stria vascularis (sv, A, B,
E, F), in lateral support cells (arrowheads in A; B), neurons of the spiral
ganglion (SG, I, K) and the auditory nerve (AN, I, K) in vehicle injected mice.
ZKV infection shows upregulation of eNOS in the Type I, Type II and Type III
fibrocytes in the spiral ligament and stria vascularis (C, D, G, H) where
immunoreactivity appears as larger aggregates. eNOS is also upregulated in
spiral ganglion neurons in a nonneuronal pattern and in the auditory nerve (J,
L). eNOS is not upregulated in the cochlear epithelium after ZIKV infection (cf
C, D to A, B). DAPI counterstains are shown (B, D, F, H, K, L). Boxed regions in
A and C shown in E and G, respectively. Scale bar for B-D in A. Scale bar for
F-H in E. Scale bar for I-L in K.