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. Author manuscript; available in PMC: 2021 Feb 24.
Published in final edited form as: Nat Cell Biol. 2020 Aug 24;22(9):1064–1075. doi: 10.1038/s41556-020-0562-4

Extended Data Fig. 2. HDAC2 mediates deacetylation of PD-L1.

Extended Data Fig. 2

a, IB analysis of WCL and anti-Flag IPs derived from 293T cells transfected with Myc-p300, HA-ins-PD-L1 and/or Flag-tagged deacetylases. b, IB analysis of WCL and Ni-NTA pull-down products from MDA-MB-231 WT and HDAC2 knockout (KO) cells transfected with His-Ub and treated with 10 μM MG-132 overnight. c, d, IB analysis of WCL derived from BT-549 PD-L1 KO cells transfected with HA-PD-L1 WT, K263R or K263Q mutants and treated with 150 μg/ml cycloheximide (CHX) for indicated hours (c). Signal intensity of PD-L1 protein was quantified by ImageJ as indicated (d). e, IB analysis of WCL and anti-Myc IPs derived from 293T cells transfected with indicated constructs. Western blots in a-c and e were performed for n=2 independent experiments with similar results. Unprocessed immunoblots are shown in Source Data Extended Data Fig. 2.