FIGURE 1.

Immunoblots comparing normal and C8 α-γ deficient sera. Western blots of C8 or patient sera were probed using rabbit polyclonal antibodies raised against C8 or C8 α-γ and developed with alkaline phosphatase labelled goat anti-rabbit IgG. Left Panel: non-reducing conditions. The position of the C8α−γ and β subunits is shown in the far-left lane. Right Panel: reducing conditions. The position of the individual C8α and C8γ polypeptide chains is shown in the far-right lane. In contrast to their presence in normal human sera (NHS), neither the C8α-γ subunit, nor the individual α and γ polypeptides was evident in the sera from C8α-γ deficient patients (Pt). Each patient’s serum was examined at least twice. In reducing gels, the bands (*) at ~55 and 25 kDa are consistent with the heavy and light chains of IgG.