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. 2020 Sep 8;202(19):e00335-20. doi: 10.1128/JB.00335-20

TABLE 2.

Plasmids used in this work

Plasmid Description Reference or source
pBJ114 Kmr galK 109
pSWU30 Tetr 63
pSW105 Kmr PpilA 66
pBADNTF pBAD24 for N-terminal FLAG fusion and with arabinose-inducible promoter, Ampr 110
pLA3 pBADNTF wcaJ Ampr 59
pSM13 pUC18, wbaP from S. enterica Ty2 containing a 1-bp deletion at position 583 a 2-bp deletion at position 645, which causes a frame shift at WbaP I194 and frame restoration at Y215, Ampr 58
pJD132 pBluescript SK, wbaP and flanking sequences from E. coli O9 : K30, Ampr 111
pWQ499 pKV102 containing rcsAK30, Tetr Chris Whitfield
pMAT150 pBJ114, in-frame deletion construct for pilT Kmr Anke Treuner-Lange
pMP001 pBJ114, in-frame deletion construct for MXAN_7415 Kmr This study
pMP012 pBJ114, in-frame deletion construct for MXAN_7421 Kmr This study
pMP015 pBJ114, in-frame deletion construct for MXAN_7442 Kmr This study
pMP016 pBJ114, in-frame deletion construct for MXAN_7416 Kmr This study
pMP018 pBJ114, in-frame deletion construct for MXAN_7417 Kmr This study
pMP021 pSWU30 Pnat MXAN_7415 Tetr This study
pMP024 pSWU30 Pnat MXAN_7416 Tetr This study
pMP030 pSW105 MXAN_7417 Kmr This study
pMP032 pSW105 MXAN_7421 Kmr This study
pMP091 pSWU30 Pnat MXAN_7442 Tetr This study
pMP124 pBJ114, in-frame deletion construct for MXAN_1043 Kmr This study
pMP146 pBADNTF MXAN_7415 Ampr This study
pJJ1 pBJ114, in-frame deletion construct for MXAN_1035 Kmr This study
pJJ2 pBJ114, in-frame deletion construct for MXAN_1025 Kmr This study
pJJ3 pBJ114, in-frame deletion construct for MXAN_1052 Kmr This study
pJJ4 pBJ114, in-frame deletion construct for MXAN_1915 Kmr This study